In silico study of macromolecular crowding effects on biochemical signaling

11 June 2013
10:15
Koichi Takahashi
Abstract

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Signal transduction pathways are sophisticated information processing machinery in the cell that is arguably taking advantage of highly non-idealistic natures of intracellular environments for its optimum operations. In this study, we focused on effects of intracellular macromolecular crowding on signal transduction pathways using single-particle simulations. We have previously shown that rebinding of kinases to substrates can remarkably increase processivity of dual-phosphorylation reactions and change both steady-state and transient responses of the reaction network. We found that molecular crowding drastically enhances the rebinding effect, and it shows nonlinear time dependency although kinetics at the macroscopic level still follows the conventional model in dilute media. We applied the rate law revised on the basis of these calculations to MEK-ERK system and compared it with experimental measurements.

***** PLEASE NOTE THAT THIS WILL TAKE PLACE ON TUESDAY 11TH JUNE ****

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