Dept of Engineering Maths University of Bristol

Biomolecular condensates are membraneless assemblies of biomolecules (such as proteins or nucleic acids) formed through liquid-liquid phase separation. Many biomolecules are electrically charged, making condensates highly sensitive to the local electrochemical environment. In this talk, I will discuss our recent theoretical work on the dynamics of charged condensates and the role of salt concentration in their evolution toward equilibrium. Two-dimensional simulations of a thermodynamically consistent phase-field model reveal that salt can arrest coarsening by affecting the relative strength of interfacial energy, associated with the condensate surface, and electrostatic energy, arising from the formation of an electric double layer across liquid interfaces. At low salt concentrations, the electrostatic energy of the double layer becomes comparable to the interfacial energy, resulting in the emergence of multiple condensates with a fixed size. These results show that salt can act as a dynamic regulator of condensate size, with implications for both understanding biological organisation and modulating the behaviour of synthetic condensates.

In this talk I shall describe recent work inspired by problems in cell biology, namely how the dynamics of small G-proteins underlies polarity formation. Their dynamics is such that their active membrane bound form diffuses more slowly. Hence you might expect Turing patterns. Yet how do cells form backs and fronts or single isolated patches. In understanding these questions we shall show that the key is to identify the parameter region where Turing bifurcations are sub-critical. What emerges is a unified 2-parameter bifurcation diagram containing pinned fronts, localised spots, localised patterns. This diagram appears in many canonical models such as Schnakenberg and Brusselator, as well as biologically more realistic systems. A link is also found between theories of semi-string interaction asymptotics and so-called homoclinic snaking. I will close with some remarks about relevance to root hair formation and to the importance of subcriticality in biology.