L3

The ability of cells to sense and respond to the mechanical properties of their environments is fundamental to cellular behaviour, with stiffness found to be a key control parameter. The physical mechanisms underpinning mechanosensing are, however, not well understood. I here consider the key physical cellular behaviours of active contractility of the internal cytoskeleton and cell growth, coupling these into mechanical models. These models suggest new distinct mechanisms of mechanotransduction in cells and tissues.

I will discuss a new theoretical approach to information and decisions in signalling systems and relate this to new experimental results about the NF-kappaB signalling system. NF-kappaB is an exemplar system that controls inflammation and in different contexts has varying effects on cell death and cell division. It is commonly claimed that it is information processing hub, taking in signals about the infection and stress status of the tissue environment and as a consequence of the oscillations, transmitting higher amounts of information to the hundreds of genes it controls. My aim is to develop a conceptual and mathematical framework to enable a rigorous quantifiable discussion of information in this context in order to follow Francis Crick's counsel that it is better in biology to follow the flow of information than those of matter or energy. In my approach the value of the information in the signalling system is defined by how well it can be used to make the "correct decisions" when those "decisions" are made by molecular networks. As part of this I will introduce a new mathematical method for the analysis and simulation of large stochastic non-linear oscillating systems. This allows an analytic analysis of the stochastic relationship between input and response and shows that for tightly-coupled systems like those based on current models for signalling systems, clocks, and the cell cycle this relationship is highly constrained and non-generic.

Locomotion strategies employed by unicellular organism are a rich source of inspiration for studying mechanisms for shape control. They are particularly interesting because they are invisible to the naked eye, and offer surprising new solutions to the question of how shape can be controlled.

In recent years, we have studied locomotion and shape control in Euglena gracilis. This unicellular protist is particularly intriguing because it can adopt different motility strategies: swimming by flagellar propulsion, or crawling thanks to large amplitude shape changes of the whole body (a behavior known as metaboly). We will survey our most recent findings within this stream of research.

A new generation of low cost 3D tomography systems is based on multiple emitters and sensors that partially convolve measurements. A successful approach to deconvolve the measurements is to use nonlinear compressed sensing models. We discuss such models, as well as algorithms for their solution. 

The peeling of an elastic sheet away from thin layer of viscous fluid is a simply-stated and generic problem, that involves complex interactions between flow and elastic deformation on a range of length scales. 

I will illustrate the possibilities by considering theoretically and experimentally the injection and spread of viscous fluid beneath a flexible elastic lid; the injected fluid forms a blister, which spreads by peeling the lid away at the  perimeter of the blister. Among the many questions to be considered are the mechanisms for relieving the elastic analogue of the contact-line problem, whether peeling is "by bending" or "by pulling", the stability of the peeling front, and the effects of a capillary meniscus when peeling is by air injection. The result is a plethora of dynamical regimes and asymptotic scaling laws.

The costs to Vodafone of calls terminating on other networks – especially fixed networks – are largely determined by the termination charges levied by other telecoms operators.  We interconnect to several other telecoms operators, who charge differently; within one interconnect operator, costs vary depending on which of their switching centres we deliver calls to, and what the terminating phone number is.  So, while these termination costs depend partly on factors that we cannot control (such as the number called, the call duration and the time of day), they are also influenced by some factors that we can control.  In particular, we can route calls within our network before handing them over from our network to the other telecoms operator; where this “handover” occurs has an impact on termination cost.  
Vodafone would like to develop a repeatable capability to determine call delivery cost efficiency and identify where network routing changes can be made to improve matters, and determine traffic growth forecasts.

Elastic gridshells arise from the buckling of an initially planar grid of rods. Architectural elastic gridshells first appeared in the 1970’s. However, to date, only a limited number of examples have been constructed around the world, primarily due to the challenges involved in their structural design. Yet, elastic gridshells are highly appealing: they can cover wide spans with low self-weight, they allow for aesthetically pleasing shapes and their construction is typically simple and rapid. A more mundane example is the classic pasta strainer, which, with its remarkably simple design, is a must-have in every kitchen.

This talk will focus on the geometry-driven nature of elastic gridshells. We use a geometric model based on the theory of discrete Chebyshev nets (originally developed for woven fabric) to rationalize their actuated shapes. Validation is provided by precision experiments and rod-based simulations. We also investigate the linear mechanical response (rigidity) and the non-local behavior of these discrete shells under point-load indentation. Combining experiments, simulations, and scaling analysis leads to a master curve that relates the structural rigidity to the underlying geometric and material properties. Our results indicate that the mechanical response of elastic gridshells, and their underlying characteristic forces, are dictated by Euler's elastica rather than by shell-related quantities. The prominence of geometry that we identify in elastic gridshells should allow for our results to transfer across length scales: from architectural structures to micro/nano–1-df mechanical actuators and self-assembly systems.

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In laboratories around the world, scientists use magnetic stirrers to mix solutions and dissolve powders. It is well known that at high drive rates the stir bar jumps around erratically with poor mixing, leading to its nick-name 'flea'. Investigating this behaviour, we discovered a state in which the flea levitates stably above the base of the vessel, supported by magnetic repulsion between flea and drive magnet. The vertical motion is oscillatory and the angular motion a superposition of rotation and oscillation. By solving the coupled vertical and angular equations of motion, we characterised the flea’s behaviour in terms of two dimensionless quantities: (i) the normalized drive speed and (ii) the ratio of magnetic to viscous forces. However, Earnshaw’s theorem states that levitation via any arrangement of static magnets is only possible with additional stabilising forces. In our system, we find that these forces arise from the flea’s oscillations which pump fluid radially outwards, and are only present for a narrow range of Reynold's numbers. At slower, creeping flow speeds, only viscous forces are present, whereas at higher speeds, the flow reverses direction and the flea is no longer stable. We also use both the levitating and non-levitating states to measure rheological properties of the system.

Tubing issues: 

- Moving a sphere in a narrow pipe

What is the force required to move an object inside a narrow elastic pipe? The constriction by the tube induces a normal force on the sphere. In the case of solid friction, the pulling force may  be simply deduced from Coulomb’s law. How does is such force modified by the addition of a lubricant? This coupled problem between elasticity and viscous flow results in a non-linear dependence of the force with the traction speed.

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- Baromorphs

When a bicycle tyre is inflated the cross section of the pipe increases much more than its circumference. Can we use this effect to induce non-isotropic growth in a plate?  We developed, through standard casting techniques, flat plates imbedded with a network of channels of controlled geometry. How are such plates deformed as pressure is applied to this network? Using a simplified mechanical model, 3D complex shapes can be programmed and dynamically actuated. 

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